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| 价格 | ￥4800.00 |
| 品牌 | NewEastBiosciences  |
| 产地 | 中国 武汉 |
| 货号 | 26468 |
| 规格 | 100μl |
| 说明书 | 说明书下载 |
RAS(K117N) [26468]
[100ul]
| Catalog Number: 26468 |
| Gene Symbol: H-Ras; K-Ras; N-Ras |
| Description: Anti RAS(K117N) Mouse Monoclonal Antibody |
| Background: The K117N mutation of KRas results in an amino acid substitution at position 146, from an alanine to a threonine. KRAS encodes a protein that is a member of the small GTPase superfamily. Ras K117N mutation results in decreased GTPase activity and constitutive signaling. It can be found in many tumors, such as lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. |
| Immunogen: A synthetic peptide from the internal region of Ras which includes the mutation of K117N, human origin. |
| Applications: ELISA, WB, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:500-1:1000 IHC: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: |
| Purity: Purified from ascites |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes K117N mutant, but not wild-type RAS of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
| Western blot: |
 |
Western blot analysis of recombinant RAS(K117N) and wild-type proteins. Purified His-tagged RAS(K117N) protein (lane 1) and corresponding wild-type protein (lane 2) were blotted with Anti RAS(K117N) monoclonal antibody (Cat. #26468). |
| Immunofluorescence: |
|
Immunofluorescence of cells expressing KRas proteins with anti-KRAS(K117N) antibody. HEK293T cells were transfected with pCDNA3-GFP-KRas (WT) plasmid (left column) or pCDNA3-GFP-KRAS(K117N) plasmid (right column), then fixed and stained with anti-KRAS(K117N) monoclonal antibody (Cat. #26468). |
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| Publications: |
| 1. DNA vaccine elicits an efficient antitumor response by targeting the mutant Kras in a transgenic mouse lung cancer model Gene Therapy 21, 888-896 (October 2014) |
| 2. Are all RAS mutations the same? Coexisting KRAS and NRAS mutations in a caecal adenocarcinoma and contiguous tubulovillous adenoma J Clin Pathol. 2015 Aug;68(8):657-60. doi: 10.1136 |
