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价格 | ¥4800.00 |
品牌 | NewEast Biosciences |
产地 | 中国 武汉 |
货号 | 26382 |
规格 | 100μl |
说明书 | 说明书下载 |
ERBB2(D769Y) [26382]
[100ul]
Catalog Number: 26382 |
Gene Symbol: ERBB2/HER2/Neu/c-Neu |
Description: Anti ERBB2(D769Y) Mouse Monoclonal Antibody |
Background: The HER-2/neu oncogene, a member of the epidermal growth factor receptor or erb-B gene-like family, encodes a transmembrane tyrosine kinase receptor that mediates extracellular signals activated by epidermal growth factors. Her2 abnormal has been strongly associated with many malignant tumors, especially with breast cancers. The expression level of Her2 is an important critiria in clinic evaluating of the progression of breast cancer. |
Immunogen: A synthetic peptide from the internal region of ERBB2 which includes the mutation of D769Y, human origin. |
Applications: ELISA, IF, IHC |
Recommended Dilutions: ELISA: 1:1000-1:2000 IF: 1:500-1:1000 IHC: 1:50-1:100 |
Concentration: 1 mg/ml |
Host: Mouse |
Format: Liquid |
Clonality: Monoclonal |
Isotype: |
Purity: Purified from ascites |
Storage buffer: |
Preservative: no |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
Species Reactivity: Recognizes D769Y mutant, but not wild-type ERBB2 of vertebrates. |
Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
Immunofluorescence: |
Immunofluorescence of cells expressing ERBB2 proteins with Anti ERBB2(D769Y) antibody. HEK293T cells were transfected with pCDNA3-GFP ERBB2(D769Y) plasmid, pCDNA3-GFP ERBB2(WT) plasmid or pCDNA3-GFP ERBB2(D769H) plasmid, then fixed and stained with Anti ERBB2(D769Y) monoclonal antibody (Cat. #26382). |
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