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Anti-BRaf(V600D) Mouse Monoclonal Antibody

价格¥4800.00
品牌NewEast Biosciences   
产地中国 武汉
货号26184
规格100ul
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  • BRaf(V600D) [26184]
    [100 µL]

     

    Catalog Number:  26184
    Gene Symbol:  BRAF, BRAF1, RAFB1
    Description:   Anti BRaf(V600D) Mouse Monoclonal Antibody
    Background:   BRaf is a member of the Raf family of Ser/Thr protein kinases. It functions downstream of Ras to regulate the MAP kinase signaling pathway. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome. Acquired mutations in BRAF have been found in cancers.
    Immunogen:   A synthetic peptide from the internal region of BRaf(V600D), human origin.
    Applications:   ELISA, WB, IF, IHC
    Recommended Dilutions:   
    ELISA 1:1000-1:5000 
    WB 1:500-1:2000 
    IHC 1:100-1:200
    Concentration:    0.5 mg/ml
    Host:   Mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   IgG
    Purity:   Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Recognizes B-RafV600D, but not wild-type BRaf proteins from vertebrates.
    Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
     
    Western blot:  
    Western blot analysis of recombinant Braf proteins. Purified His-tagged BRaf(V600D) protein (amino acids 513-693, lane 2) and corresponding wild type protein (lane 1) were blotted with Anti BRaf(V600D) monoclonal antibody (Cat. # 26184).
    Immunofluorescence:   
    Immunofluorescence of cells expressing BRaf proteins with Anti BRaf(V600D) antibody. HEK293T cells were transfected with pCDNA3-GFP BRaf(WT) plasmid (left column) or pCDNA3-GFP BRaf(V600D) plasmid (right column), then fixed and stained with Anti BRaf(V600D) monoclonal antibody (Cat. #26184).
    Related Products
    Catalog# Name Size Price
    21271 Anti BRaf Rabbit Polyclonal Antibody  100 μL  ¥2600   
    26129 Anti BRaf Mouse Monoclonal Antibody  100 μL  ¥2800 
    26109 Anti BRaf(N581S) Mouse Monoclonal Antibody  100 μL  ¥4800
    26039 Anti BRaf(V600E) Mouse Monoclonal Antibody  100 μL  ¥4800
    26110 Anti BRaf(V600K) Mouse Monoclonal Antibody  100 μL  ¥4800
    26184 Anti BRaf(V600D) Mouse Monoclonal Antibody  100 μL  ¥4800
    26182 Anti BRaf(V600R) Mouse Monoclonal Antibody  100 μL  ¥4800
    26196 Anti BRaf(G469E) Mouse Monoclonal Antibody  100 μL  ¥4800

     

     

     

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