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| 价格 | ￥4800.00 |
| 品牌 | NewEast Biosciences  |
| 产地 | 中国 武汉 |
| 货号 | 26242 |
| 规格 | 100ul |
| 说明书 | 说明书下载 |
EML4-ALK [26242]
[100ul]
| Catalog Number: 26242 |
| Gene Symbol: EML4-ALK |
| Description: Anti EML4 ALK Mouse Monoclonal Antibody |
| Background: EML4-ALK is a fusion-type protein kinase that can be found in many tumors, such as anaplastic large cell lymphoma, inflammatory myofibroblastic tumor, neuroblastoma and NSCLC, and so on. It is generated as a result of a small inversion within the short arm of human chromosome 2. EML4-ALK protein often promotes and maintains the malignant behavior of the cancer cells by activating the MAPK, PI3K/AKT and JAK/STAT3 pathways. |
| Immunogen: A synthetic peptide from the internal region of EML4-ALK of human origin. |
| Applications: ELISA, WB, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:2000 WB: 1:500-1:1000 IHC: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: |
| Purity: Purified from ascites |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes T41A mutant, but not wild-type EML4-ALK of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
| Western blot: |
 |
Western blot analysis of recombinant EML4 ALK and wildtype proteins. Purified His-tagged EML4-ALK protein (lane 3), corresponding wild-type EML4 protein (lane1) and wild-type ALK protein (lane2) were blotted with Anti EML4 ALK monoclonal antibody (Cat. #26242). |
| Immunofluorescence: |
|
Immunofluorescence of cells expressing EML4-ALK proteins with Anti EML4 ALK antibody. HEK293T cells were transfected with pCDNA3-GFP-EML4 (WT) plasmid (left column) or pCDNA3-GFP ALK(WT) plasmid (middle column) or pCDNA3-GFP-EML4-ALK plasmid (right column), then fixed and stained with Anti EML4 ALK monoclonal antibody (Cat. #26242). |
| Related Products | |||
| Catalog# | Name | Size | Price |
| 21280 | Anti ALK Rabbit Polyclonal Antibody | 100 μL | ￥2,600 |
| 26242 | Anti EML4 ALK Mouse Monoclonal Antibody | 100 μL | ￥4,800 |
| 26290 | Anti ALK(D1225N) Mouse Monoclonal Antibody | 100 μL | ￥4,800 |
| 26226 | Anti ALK(C1156Y) Mouse Monoclonal Antibody | 100 μL | ￥4,800 |
| 26151 | Anti ALK(R1275Q) Mouse Monoclonal Antibody | 100 μL | ￥4,800 |
