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| 价格 | ￥4800.00 |
| 品牌 | NewEastBiosciences  |
| 产地 | 中国 武汉 |
| 货号 | 26191 |
| 规格 | 100ul |
| 说明书 | 说明书下载 |
Ras(G13A) [26191]
[100ul]
| Catalog Number: 26191 |
| Gene Symbol: HRas; KRas; NRas |
| Description: Anti HRAS(G13A) Mouse Monoclonal Antibody |
| Background: Belongs to the small GTPase superfamily. Ras family binds GDP/GTP and possess intrinsic GTPase activity, alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP).Located in cell membrane, lipid-anchor, cytoplasmic side and Golgi apparatus membrane. |
| Immunogen: A synthetic peptide from the internal region of HRas which includes the mutation of G13A, human origin. |
| Applications: ELISA, WB, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:500-1:1000 IHC: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: |
| Purity: Purified from ascites |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes G13A mutant, but not wild-type H-RAS of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
| Western blot: |
 |
Western blot analysis of recombinant HRAS(G13A) and wildtype proteins. Purified His-tagged HRAS(G13A) protein (lane 2) and corresponding wildtype protein (lane 1) were blotted with Anti HRAS(G13A) monoclonal antibody (Cat. #26191). |
| Immunofluorescence: |
|
Immunofluorescence of cells expressing Ras proteins with Anti HRAS(G13A) antibody. HEK293T cells were transfected with pCDNA3-GFP HRas(WT) plasmid (left column) or pCDNA3-GFP HRAS(G13A) plasmid (right column), then fixed and stained with Anti HRAS(G13A) monoclonal antibody (Cat. #26191). |
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| Publications: |
| 1. DNA vaccine elicits an efficient antitumor response by targeting the mutant Kras in a transgenic mouse lung cancer model Gene Therapy 21, 888-896 (October 2014) |
| 2. Are all RAS mutations the same? Coexisting KRAS and NRAS mutations in a caecal adenocarcinoma and contiguous tubulovillous adenoma J Clin Pathol. 2015 Aug;68(8):657-60. doi: 10.1136 |
