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Anti- ERBB2 Mouse Monoclonal Antibody

价格¥2200.00
品牌NewEast Biosciences   
产地中国 武汉
货号26127
规格100ul
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  • ERBB2 [26127]
    [100 µL]

     

    Catalog Number:  26127
    Gene Symbol:  ERBB2/HER2/Neu/c-Neu
    Description:   Anti ERBB2 Mouse Monoclonal Antibody
    Background:   The HER-2/neu oncogene, a member of the epidermal growth factor receptor or erb-B gene-like family, encodes a transmembrane tyrosine kinase receptor that mediates extracellular signals activated by epidermal growth factors. Her2 abnormal has been strongly associated with many malignant tumors, especially with breast cancers. The expression level of Her2 is an important critiria in clinic evaluating of the progression of breast cancer.
    Immunogen:   A synthetic peptide from the C-terminal region of ERBB2, human origin
    Applications:   ELISA, WB, IHC
    Recommended Dilutions:   
    ELISA: 1:1000-1:5000 
    WB: 1:500-1:2000 
    IHC: 1:50-1:200
    Concentration:    1mg/ml
    Host:   Mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   
    Purity:   Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:   Recognizes Erb-B2 of vertebrates.
    Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
     
    Western blot:  
    Western blot analysis of recombinant Erb-B2 protein. Purified His-tagged Erb-B2 protein (Cat. #10208) was blotted with anti ErbB2 monoclonal antibody (Cat. #26127).
    Immunohistochemistry:    
    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with anti-Erb-B2 monoclonal antibody (Cat. #26127). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (1:50 dilution) was used as secondary antibody.

     

    Related Products
    Catalog# Name Size Price
    21200 Anti ERBB2 Rabbit polyclonal Antibody  100 μL  ¥2600 
    21202 Anti ERBB2 Rabbit Polyclonal Antibody  100 μL  ¥2600   
    26080 Anti ERBB2(V777A) mouse monoclonal Antibody  100 μL  ¥4800   
    26127 Anti ERBB2 mouse monoclonal Antibody  100 μL  ¥2800  

     

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