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Anti EGFR(D761Y) Mouse Monoclonal Antibody

价格¥4800.00
品牌NewEast Biosciences   
产地中国 武汉
货号26120
规格100ul
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  • EGFR(D761Y) [26120]
    [100 µL]

     

    Catalog Number:  26120
    Gene Symbol:  EGFR/HER1/ERBB1
    Description:   Anti EGFR(D761Y) Mouse Monoclonal Antibody
    Background:   EGFR is a receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. It activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. Defects in EGFR are associated with lung cancer (LNCR).
    Immunogen:   A synthetic peptide from the internal region of EGFR which includes the mutation of D761Y, human origin.
    Tested Applications:   ELISA, WB, IF, IHC
    Recommended Dilutions:   
    ELISA: 1:1000-1:5000 
    WB: 1:500-1:2000 
    IHC: 1:50-1:100
    Concentration:   1 mg/ml
    Host:   mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   
    Purity:   Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Recognizes EGFR(D761Y), but not wild type EGFR from vertebrates.
    Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles.
     
    Western blot:  
    Western blot analysis of recombinant EGFR(D761Y) and wildtype proteins. Purified His-tagged EGFR(D761Y) protein (amino acids 702-987, lane 2, Cat. # 10240) and corresponding wild type protein (lane 1, Cat. #10210) were blotted with anti EGFR(D761Y) mouse monoclonal antibody (Cat. #26120). Proteins loading (bottom) were blotted with anti-His-tag monoclonal antibody (Cat. #28004).
    Immunofluorescence:  
    Immunofluorescence of cells expressing EGFR proteins with anti EGFR(D761Y) antibody. HEK293T cells were transfected with pCDNA3-GFP EGFR(WT) plasmid (left column) or pCDNA3-GFP EGFR(D761Y) plasmid (right column), then fixed and stained with anti EGFR(D761Y) monoclonal antibody (Cat. #26120).

     

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