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Anti-TP53(R175H) Mouse Monoclonal Antibody

价格¥4800.00
品牌NewEast Biosciences   
产地中国 武汉
货号26072
规格100ul
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  • TP53(R175H) mono [26072]
    [100 µL]

    Catalog Number:  26072
    Gene Symbol:  TP53(R175H)
    Description:    Anti TP53(R175H) Mouse Monoclonal Antibody
    Background:   TP53 belongs to the p53 proteins family. It is ubiquitously expressed, acts as a tumor suppressor in many tumor types and induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Defects in TP53 are a cause of esophageal cancer (ESCR), lung cancer (LNCR), adrenocortical carcinoma (ADCC), choroid plexus papilloma (CPLPA) and so on.
    Immunogen:   A synthetic peptide from the internal region of TP53 which includes the mutation of R175H, human origin
    Applications:   ELISA, WB, IHC
    Recommended Dilutions:   
    ELISA: 1:1000-1:5000 
    WB: 1:1000-1:1000 
    IHC: 1:50-1:100
    Concentration:    1 mg/ml
    Host:   mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   
    Purity:   Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Anti TP53(R175H) antibody recognizes TP53(R175H) of vertebrates.
    Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles.
     
    Western blot:  
    Western blot analysis of recombinant TP53(R175H) and wildtype proteins. Purified His-tagged TP53(R175H) protein(Cat.#10207) and wildtype protein(Cat.#10202) were blotted with Anti TP53(R175H) monoclonal antibody(Cat.# 26072).
    Immunofluorescence:    
    Immunofluorescence of cells expressing TP53 proteins with anti TP53(R175H) antibody. HEK293T cells were transfected with pCDNA3-GFP TP53(WT) plasmid (left column) or pCDNA3-GFP TP53(R175H) plasmid (right column), then fixed and stained with anti- TP53(R175H) monoclonal antibody (Cat. # 26072).

     

    Related Products
    Catalog# Name Size Price
    21270 Anti TP53 Rabbit Polyclonal Antibody 100 μL ¥2,800
    26076 Anti TP53(R282W) Mouse Monoclonal Antibody 100 μL  ¥4800
    26072 Anti TP53(R175H) Mouse Monoclonal Antibody 100 μL  ¥4800
    21165 Anti TP53(R282W) Rabbit Polyclonal Antibody 100 μL  ¥4800
    21161 Anti TP53(R175H) Rabbit Polyclonal Antibody 100 μL  ¥4800

     

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