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| 价格 | ￥1600.00 |
| 品牌 | NewEast Biosciences  |
| 产地 | 中国 武汉 |
| 货号 | 26136 |
| 规格 | 100μl |
| 说明书 | 说明书下载 |
IDH1 [26136]
[100 µL]
| Catalog Number: 26136 |
| Gene Symbol: IDH1 |
| Description: Anti IDH1 Mouse Monoclonal Antibody |
| Background: Isocitrate dehydrogenase (IDH) catalyzes the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Mutations of Arg-132 in IDH to Cys, His, Leu or Ser abolish magnesium binding and the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R-2-hydroxyglutarate. Elevated levels of R-2-hydroxyglutarate are correlated with an elevated risk of malignant brain tumors. |
| Immunogen: Recombinant proteins of human IDH1 (amino acids 1-240). |
| Applications: ELISA, WB, IHC, IP |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:100-1:1000 IHC: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host: mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG |
| Purity: Purified from ascites |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Anti IDH1 antibody recognizes IDH1 of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
| Western blot: |
 |
Western blot analysis of recombinant IDH1 protein. Purified His-tagged IDH1 protein (amino acids 1-240) (Cat.#10198) was blotted with anti IDH1 mouse antibody (Cat.# 26136). |
| Related Products | |||
| Catalog# | Name | Size | Price |
| 26136 | Anti IDH1 Mouse Monoclonal Antibody | 100 μL | ￥4800 |
| 26406 | Anti IDH1(R132P) Mouse Monoclonal Antibody | 100 μL | ￥4,800 |
| 26160 | Anti IDH1(R132S) Mouse Monoclonal Antibody | 100 μL | ￥4,800 |
| 26081 | Anti IDH1(R132H) Mouse Monoclonal Antibody | 100 μL | ￥4800 |
| Publications: |
| 1. Specific monoclonal antibodies against IDH1/2 mutations as diagnostic tools for gliomas Brain Tumor Pathology. January 2015, Volume 32, Issue 1, pp 3-11 |
| 2. Mutant IDH inhibits HNF-4α to block hepatocyte differentiation and promote biliary cancer Nature 513, 110–114 (04 September 2014) |
| 3. Metabolomic comparison between cells overexpressing isocitrate dehydrogenase 1 and 2 mutants and the effects of an inhibitor on the metabolism Journal of Neurochemistry. Volume 132, Issue 2, pages 183–193, January 2015 |
