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Anti-PIK3CA(E545K) Rabbit Polyclonal Antibody

价格¥4800.00
品牌NewEast Biosciences   
产地中国 武汉
货号21145
规格100ul
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  • PIK3CA(E545K) [21145]
    [100 µL]

     

    Catalog Number:  21145
    Gene Symbol:  PIK3CA(E545K)
    Description:   Anti PIK3CA(E545K) Rabbit Polyclonal Antibody
    Background:   Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The catalytic subunit is encoded by the PIK3CA gene. It phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2. The PIK3CA gene is one of the most frequently mutated genes in human cancers.
    Immunogen:   A synthetic peptide from the internal region of PIK3CA(E545K) of human origin.
    Applications:   ELISA, WB, IHC, IP
    Recommended Dilutions:   
    ELISA: 1:100-1:5000 
    WB: 1:100-1:1000 
    IHC: 1:10-1:100
    Concentration:   0.2 mg/ml
    Host:   Rabbit
    Format:   Liquid
    Clonality:   Polyclonal
    Purity:   Purified from serum
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Anti PIK3CA(E545K) antibody recognizes PIK3CA(E545K) of vertebrates.
    Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
     
    Western blot:  
    Western blot analysis of PIK3CA wild type and E545K proteins. Equal amounts of purified PIK3CA(E545K)(amino acids 461-640) and corresponding wild type proteins were loaded and blotted with Anti PIK3CA(E545K) rabbit polyclonal antibody (Cat. # 21145).

     

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