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Anti-EYA2 Mouse Monoclonal Antibody ( protein)

价格1600.00
品牌NewEast Biosciences   
产地中国 武汉
货号26096
规格100ul
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  • EYA2 [26096]
    [100 µL]

     

    Catalog Number:  26096
    Gene Symbol:  EYA2
    Description:   Anti EYA2 Mouse Monoclonal Antibody
    Background:   EYA2 may be involved in development of the eye. It promotes efficient DNA repair by dephosphorylating H2AX, promoting the recruitment of DNA repair complexes containing MDC1. Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis. EYA2 coactivates SIX1 and seems to coactivate SIX2, SIX4 and SIX5. It seems to be involved in myogenesis together with SIX1 and DACH2.
    Immunogen:   Human EYA2 protein
    Applications:   ELISA, WB, IHC
    Recommended Dilutions:    
    ELISA: 1:1000-1:5000 
    WB: 1:100-1:1000 
    IHC: 1:50-1:100
    Concentration:   0.8 mg/ml
    Host:   mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   
    Purity:   Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg 2+ and Ca 2+ ), pH 7.4, 150 mM NaCl, 50% glycerol Species
    Species Reactivity:  Anti EYA2 antibody recognizes EYA2 of vertebrates.
    Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles.
     
    Western blot:  
    Western blot analysis of recombinant full length EYA2 protein. Purified His-tagged EYA2 protein(Cat. #10195) was blotted with anti EYA2 monoclonal antibody (Cat # 26096).
    Immunohistochemistry:    
    Immunohistochemical analysis of paraffin-embedded thyroid cancer tissue with anti EYA2 monoclonal antibody (Cat. #26096). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (dilution 1:50) was used as secondary antibody.

     

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