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价格 | ¥4800.00 |
品牌 | NewEast Biosciences |
产地 | 中国 武汉 |
货号 | 26903 |
规格 | 30μl |
说明书 | 说明书下载 |
Active Rac1 GTP [26903] [30 µL] |
Catalog Number: 26903 |
Gene Symbol: RAC1 |
Description: Anti Active Rac1-GTP Mouse Monoclonal Antibody |
Background: Small GTPases are a super-family of cellular signaling regulators. Rac belongs to the Rho sub-family of GTPases that regulate cell motility, cell division, and gene transcription. GTP binding increases the activity of Rac, and the hydrolysis of GTP to GDP renders it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs). |
Immunogen: Recombinant full length protein of active Rac1 |
Applications: IP, IHC |
Recommended Dilutions: 1 µg for 1~2 mg total cellular proteins for IP; 1:100 dilution for IHC |
Concentration: 1 mg/ml |
Host: mouse |
Format: Liquid |
Clonality: Monoclonal |
Isotype: IgM |
Purity: Purified from ascites |
Storage buffer: |
Preservative: no |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
Species Reactivity: Anti-active Rac1 antibody recognizes active Rac1 from vertebrates. |
Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
Immunoprecipitation/Western blot: |
Rac activation assay. MEF cells were treated with (lane 2) or without (lane 1) PDGF. Cell lysates were incubated with an anti active Rac monoclonal antibody (Cat # 26903) (top panel). Th precipitated active Rac was immunoblotted with an anti Rac rabbit polyclonal antibody (Cat # 21003). The bottom panel shows the Western blot with anti Rac of the cell lysates used (5% of that used in the top panel). |
Immunofluorescence: |
Immunofluorescence for the active Rac using Anti Active Rac1 GTP Mouse Monoclonal Antibody [26903] shows Rac GTP immunolabeling (green) in combination with cofilin (red) on brain tissue sections. The tissue sections were fixed with -20 ºC methanol or 4% paraformaldehyde (fixation time 1hr) and stained with antibody at 1:1000 in 0.1M Phosphate buffer with 0.3% Triton X, and 4% BSA for 24h at room temperature. Secondary antibodies were anti mouse AlexaFluor488 and anti rabbit AlexaFluor594 at 1:1000. |
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发表时间:2022年
名称/货号:Anti- Active Rac1 Mouse Monoclonal Antibody/ 26903
影响因子:Cell Death & Disease IF=9
应用范围:IP
种属:Mouse
发表时间:2022年
名称/货号:Rac Pulldown Activation Assay Kit/ 80501
影响因子: Journal of Cell Communication and Signaling IF=4.1
应用范围:IP-WB
种属:Human
发表时间:2022年
名称/货号:Anti- Active Rac1 Mouse Monoclonal Antibody/26903
影响因子:Journal of Neuroinflammation IF=9.58
应用范围:IP
种属:Rat
发表时间:2022年
名称/货号:Anti- Active Rac1 Mouse Monoclonal Antibody/ 26903
影响因子:Journal of Clinical and Translational Hepatology IF=3.6
应用范围:IP
种属:Mouse
发表时间:2022年
名称/货号:Anti- Active Rac1 Mouse Monoclonal Antibody/26903
影响因子:Cell Death Discovery IF=7.1
应用范围:IP
种属:Mouse
发表时间:2022年
名称/货号:Anti- Active Rac1 Mouse Monoclonal Antibody/ 26903
影响因子:Journal of Cell Communication and Signaling IF=4.1
应用范围:IP
种属:Mouse
发表时间:2022年
名称/货号:Anti- Active Rac1 Mouse Monoclonal Antibody/26903
影响因子:Exp Cell Res IF=3.7
应用范围:IP
种属:Human
发表时间:2017年
名称/货号:Rac Pulldown Activation Assay Kit/ 80501
影响因子:Neuropsychopharmacology IF=7.16
应用范围:IP
种属:Human
发表时间:2018年
名称/货号:Anti- Active Rac1 Mouse Monoclonal Antibody/ 26903
影响因子:Acta Neuropathologica Communications IF=7.801
应用范围:IHC
种属:Mouse
发表时间:2019 年
名称/货号:Anti- Active Rac1 Mouse Monoclonal Antibody/ 26903
影响因子:Oncogene IF=7.971
应用范围:IP
种属:Human
发表时间:2017年
名称/货号:Anti- Active Rac1 Mouse Monoclonal Antibody /26903
影响因子:Kidney International IF=8.945
应用范围:IF
种属:Mouse
发表时间:2021年
名称/货号:Anti- Active Rac1 Mouse Monoclonal Antibody/ 26903
影响因子:Angiogenesis IF=9.596
应用范围:IP
种属:Human
发表时间:2020年
名称/货号:Anti- Active Rac1 Mouse Monoclonal Antibody/ 26903
影响因子:Angiogenesis IF=9.78
应用范围:IP
种属:Human Mouse
发表时间:2021 年
名称/货号:Anti- Active Rac1 Mouse Monoclonal Antibody/26903
影响因子:Advanced Healthcare Materials IF=9.933
应用范围:IHC
种属:Human
发表时间:2020年
名称/货号:Anti- Active Rac1 Mouse Monoclonal Antibody/ 26903
影响因子:Developmental Cell IF=10.092
应用范围:IP
种属: Mouse
发表时间:2017年
名称/货号:Anti- Active Rac1 Mouse Monoclonal Antibody /26903
影响因子:Journal Of Allergy And Clinical Immunology IF=10.228
应用范围:IP IF
种属:Human Mouse
发表时间:2018年
名称/货号:Rac Pulldown Activation Assay Kit /80501
影响因子:Cardiovascular Research IF=10.878
应用范围:IP-WB
种属:
发表时间:2019年
名称/货号:Anti- Active Rac1 Mouse Monoclonal Antibody /26903
影响因子:Nature Communications IF=11.878
应用范围:FC
种属:Mouse
发表时间:2019年
名称/货号:Anti- Active Rac1 Mouse Monoclonal Antibody/ 26903
影响因子:Nature Communications IF=12.121
应用范围:IP
种属:Mouse
发表时间:2020年
名称/货号:Anti- Active Rac1 Mouse Monoclonal Antibody/ 26903
影响因子:Nature Communications IF=12.121
应用范围:IP
种属:Human
发表时间:2019年
名称/货号:Anti- Active Rac1 Mouse Monoclonal Antibody /26903
影响因子:Nature Communications IF=14.919
应用范围:IHC
种属:Xenopus
发表时间:2021年
名称/货号:Anti- Active Rac1 Mouse Monoclonal Antibody /26903
影响因子:Nature Communications IF=14.919
应用范围:IHC
种属:Mouse
发表时间:2017年
名称/货号:Anti- Active Rac1 Mouse Monoclonal Antibody /26903
影响因子:Blood IF=22.113
应用范围:FC
种属:Mouse
发表时间:2017 年
名称/货号:Anti- Active Rac1 Mouse Monoclonal Antibody /26903
影响因子:Nature Immunology IF=23.53
应用范围:IF
种属:Mouse Human
发表时间:2018年
名称/货号:Anti- Active Rac1 Mouse Monoclonal Antibody /26903
影响因子:Cancer Discovery IF=29.497
应用范围:IHC
种属:Human
发表时间:2021年
名称/货号:Anti- Active Rac1 Mouse Monoclonal Antibody
影响因子:Cell IF=41.582
应用范围:IHC
种属:Mouse