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Anti- Active Rheb Mouse Monoclonal Antibody

价格￥4800.00
品牌NewEast Biosciences   
产地中国 武汉
货号26910
规格30μl
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  • 文献引用

  • 概述

    Active Rheb GTP [26910]
    [30 µL]

    Catalog Number:  26910
    Gene Symbol:  RHEB
    Description:   Anti Active Rheb Mouse Monoclonal Antibody
    Background:   Small GTPases are a super-family of cellular signaling regulators. Rheb is a member of the Ras-superfamily GTPases. Rheb has been shown to interact with C-Raf, Mammalian target of rapamycin (mTOR), TSC2, Ataxia telangiectasia mutated (ATM), KIAA1303 and Ataxia telangiectasia and Rad3 related (ATR).
    Immunogen:   Recombinant full length protein of active Rheb.
    Applications:   IP, IHC
    Recommended Dilutions:   1 μg for 1~2 mg total cellular proteins for IP
    1:100 dilution for IHC
    Concentration:   1 mg/ml
    Host:   mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   IgM
    Purity:   Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Anti active Rheb antibody recognizes active Rheb of vertebrates.
    Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
     
    Immunoprecipitation/Western blot:  
    Rheb activation assay. Purified Rheb proteins were immunoprecipitated after treated with GDP (lane 1) or GTPγS (lane 2). Immunoprecipitation was done with the anti active Rheb monoclonal antibody (Cat. No. 26910). Immunoblot was with an anti Rheb rabbit polyclonal antibody (Cat. No. 21098).
    Related Products
    Catalog# Name Size Price
    26910 Active RheB GTP Monoclonal Antibody  30 μL  ￥4800
    81201 RheB Activation Assay Kit  20 assays  ￥6800
    26015 Anti RheB Mouse Monoclonal Antibody  100 μL  ￥2800
    21098 Anti RheB Rabbit Polyclonal Antibody  100 μL  ￥2600
    Publications:
    1.  Inducible Nitric Oxide Synthase Drives mTOR Pathway Activation and Proliferation of Human Melanoma by Reversible Nitrosylation of TSC2
        Cancer Res. 2014 Feb 15;74(4):1067-78
    2.  SynGAP Regulates Protein Synthesis and Homeostatic Synaptic Plasticity in Developing Cortical Networks
        PLoS One. 2013 Dec 31;8(12):e83941