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Anti -Active RhoA Mouse Monoclonal Antibody

价格￥4800.00
品牌NewEast Biosciences   
产地中国 武汉
货号26904
规格30μl
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  • 概述

    Active RhoA GTP [26904]
    [30 µL]

     

     

    Catalog Number:  26904
    Gene Symbol:   RHOA
    Description:   Anti Active RhoA Mouse Monoclonal Antibody
    Background:   Small GTPases are a super-family of cellular signaling regulators. RhoA belongs to the Rho sub-family of GTPases. Rho proteins play critical roles in many actin cytoskeleton- dependent processes including platelet aggregation, cell motility, contraction, and cytokinesis. It regulates the formation of stress fibers and focal adhesions in fibroblasts and Ca 2+ sensitivity of smooth muscle contraction.
    Immunogen:   Recombinant full length protein of active RhoA
    Applications:   IP, IHC
    Recommended Dilutions:   1 µg for 1~2 mg total cellular proteins for IP; 
                                                       1:100 dilution for IHC
    Concentration:   1 mg/ml
    Host:   Mouse
    Format:   Liquid
    Clonality:  Monoclonal
    Isotype:   IgG2b
    Purity:   Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg 2+ and Ca 2+ ), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Anti-active RhoA antibody recognizes active RhoA from vertebrates.
    Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
     
    Immunoprecipitation/Western Blot:  

    IP/WB analysis of active RhoA protein. Purified recombinant RhoA proteins wer
    e loaded with GDP (lane 1) or GTPγS (lane 2). These proteins were immunoprecipitated
    with anti active RhoA mouse monoclonal antibody (Cat. # 26904). After SDS/PAGE, the
    membrane filter was probed with anti RhoA mouse monoclonal antibody (Cat. # 26007).

     

     

     

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