| 价格 | ￥4800.00 |
| 品牌 | NewEast Biosciences  |
| 产地 | 中国 武汉 |
| 货号 | 26184 |
| 规格 | 100ul |
| 说明书 | 说明书下载 |
BRaf(V600D) [26184]
[100 µL]
| Catalog Number: 26184 |
| Gene Symbol: BRAF, BRAF1, RAFB1 |
| Description: Anti BRaf(V600D) Mouse Monoclonal Antibody |
| Background: BRaf is a member of the Raf family of Ser/Thr protein kinases. It functions downstream of Ras to regulate the MAP kinase signaling pathway. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome. Acquired mutations in BRAF have been found in cancers. |
| Immunogen: A synthetic peptide from the internal region of BRaf(V600D), human origin. |
| Applications: ELISA, WB, IF, IHC |
| Recommended Dilutions: ELISA 1:1000-1:5000 WB 1:500-1:2000 IHC 1:100-1:200 |
| Concentration: 0.5 mg/ml |
| Host: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG |
| Purity: Purified from ascites |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes B-RafV600D, but not wild-type BRaf proteins from vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
| Western blot: |
 |
Western blot analysis of recombinant Braf proteins. Purified His-tagged BRaf(V600D) protein (amino acids 513-693, lane 2) and corresponding wild type protein (lane 1) were blotted with Anti BRaf(V600D) monoclonal antibody (Cat. # 26184). |
| Immunofluorescence: |
|
Immunofluorescence of cells expressing BRaf proteins with Anti BRaf(V600D) antibody. HEK293T cells were transfected with pCDNA3-GFP BRaf(WT) plasmid (left column) or pCDNA3-GFP BRaf(V600D) plasmid (right column), then fixed and stained with Anti BRaf(V600D) monoclonal antibody (Cat. #26184). |
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