| 价格 | ￥4800.00 |
| 品牌 | NewEast Biosciences  |
| 产地 | 中国 武汉 |
| 货号 | 26167 |
| 规格 | 100ul |
| 说明书 | 说明书下载 |
β catenin(E53K) [26167]
[100 µL]
| Catalog Number: 26167 |
| Gene Symbol: CTNNB1, CTNNB |
| Description: Anti- β- catenin(E53K) Mouse Monoclonal Antibody |
| Background: Beta-catenin (or β catenin) is a protein that in humans is encoded by the CTNNB1 gene. β-catenin is a subunit of the cadherin protein complex and also acts as an intracellular signal transducer in the Wnt signaling pathway. Deregulation of beta-catenin signaling is an important event in the genesis of a number of malignancies, such as colon cancer, melanoma, hepatocellular carcinoma, ovarian cancer, endometrial cancer, medulloblastoma pilomatricomas, and prostate cancer. |
| Immunogen: A synthetic peptide from the internal region of β catenin which includes the mutation of E53K, human origin. |
| Applications: ELISA, WB, IF, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:500-1:2000 IHC: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: |
| Purity: Purified from ascites |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes β catenin(E53K), but not wild type β catenin protein from vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
Download Datasheet:  |
| Western blot: |
|
Western blot analysis of recombinant β catenin(E53K) and wildtype proteins. Purified His-tagged β-catenin(E53K) protein (amino acids 1-76, lane 2) and corresponding wild type protein (lane 1) were blotted with anti β catenin(E53K) mouse monoclonal antibody (Cat. #26167). |
| Immunofluorescence: |
|
Immunofluorescence of cells expressing β catenin proteins with anti-β catenin(E53K) antibody. HEK293T cells were transfected with pCDNA3-GFP- β catenin WT) plasmid (left column) or pCDNA3-GFP-β catenin(E53K) plasmid (right column), then fixed and stained with anti β catenin(E53K) monoclonal antibody (Cat. #26167). |
| Related Products | |||
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| 26167 | Anti β catenin (E53K) Mouse Monoclonal Antibody | 100 μL | ￥4800 |
| 26166 | Anti β catenin (G48D) Mouse Monoclonal Antibody | 100 μL | ￥4800 |
| 26169 | Anti β catenin (G38D) Mouse Monoclonal Antibody | 100 μL | ￥4800 |
| 26170 | Anti β catenin (A43V) Mouse Monoclonal Antibody | 100 μL | ￥4800 |
| 21282 | Anti β catenin Rabbit Polyclonal Antibody | 100 μL | ￥2600 |
