Anti-TP53(R282W) Mouse Monoclonal Antibody

价格¥4800.00
品牌NewEast Bisciences   
产地中国 武汉
货号26076
规格100ul
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    TP53(R282W) mono [26076]
    [100 µL]

     

    Catalog Number:  26076
    Gene Symbol:  TP53
    Description:   Anti TP53(R282W) Mouse Monoclonal Antibody
    Background:   p53 protein acts as a tumor suppressor in many tumor types and induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Defects in TP53 are found in many types of cancers.
    Immunogen:   Recombinant p53(R282W) protein, human origin.
    Applications:   ELISA, WB, IHC, IP
    Recommended Dilutions:   
    ELISA: 1:1000-1:5000 
    WB: 1:1000-1:5000 
    IHC: 1:50-1:100
    Concentration:   1 mg/ml
    Host:   mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   IgG1
    Purity:   Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Anti TP53(R282W) antibody recognizes TP53(R282W) of vertebrates.
    Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
     
    Western blot:  
    Western blot analysis of recombinant TP53(R282W) and wild-type p53 proteins. Purified His-tagged TP53(R282W) protein (lane 2, Cat.#10204) and wild type p53 protein (lane 1, Cat.#10202) were blotted with anti TP53(R282W) mouse monoclonal antibody (Cat. # 26076).
    Immunofluorescence:    
    Immunofluorescence of cells expressing TP53 proteins with anti TP53(R282W) antibody. HEK293T cells were transfected with pCDNA3-GFP TP53(WT) plasmid (left column) or pCDNA3-GFP-TP53(R282W) plasmid (right column), then fixed and stained with anti TP53(R282W) monoclonal antibody (Cat. # 26076).

     

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