| 价格 | ￥4800.00 |
| 品牌 | NewEast Bioscienes  |
| 产地 | 中国 武汉 |
| 货号 | 26039 |
| 规格 | 100ul |
| 说明书 | 说明书下载 |
BRaf(V600E) [26039]
[100 µL]
| Catalog Number: 26039 |
| Gene Symbol: BRaf |
| Description: Anti BRaf(V600E) Mouse Monoclonal Antibody |
| Background: BRaf is a member of the Raf family of Ser/Thr protein kinases. It functions downstream of Ras to regulate the MAP kinase signaling pathway. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome. Acquired mutations in BRAF have been found in cancers |
| Immunogen: A synthetic peptide from the internal region of BRaf(V600E), human origin. |
| Applications: ELISA, WB, IHC, IP |
| Recommended Dilutions: ELISA: 1:100-1:5000 WB: 1:100-1:2000 IHC: 1:100-1:1000 |
| Concentration: 1 mg/ml |
| Host: mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG2b |
| Purity: Purified from ascites |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes BRaf(V600E), but not wild type BRaf proteins from vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
| Western blot: |
 |
Western blot analysis of recombinant Braf proteins with anti-BRaf(V600E) antibody. Purified His - tagged BRaf(V600E ) ?amino acids 513-693)(lane1) ? corresponding wild type protein(lane 2), His - tagged BRaf(V600D ) (lane 3) , His - tagged BRaf(V600A ) (lane 4) , His - tagged BRaf(V600G ) (lane 5) , His - tagged BRaf(V600K ) (lane 6), His - tagged BRaf(V600R ) (lane 7), and His - tagged BRaf( V600M ) (lane 8) were blotted with anti BRaf(V600E) monoclonal antibody (Cat # 26039) . |
| Immunofluorescence: |
|
|
| Immunofluorescence of cell expressing BRaf protein with anti BRaf(V600E) antibody . HEK293T cells were transfected with pCDNA3-GFP BRaf(V600E )plasmid,pCDNA3-GFP BRaf( WT )plasmid, pCDNA3-GFP BRaf(V600K )plasmid,pCDNA3-GFP BRaf(V600R )plasmid, pCDNA3-GFP- BRaf(V600A )plasmid,pCDNA3-GFP BRaf(V600D ) plasmid, pCDNA3-GFP-BRaf(V600M)plasmid, or pCDNA3-GFP-B-Raf(V600G)plasmid, then fixed and stained with anti BRaf(V600E) monoclonal antibody (Cat. # 26039). |
| Immunohistochemistry: |
|
Immunohistochemical analysis of paraffin-embedded human melanoma tissue with anti BRaf(V600E) monoclonal antibody (Cat. # 26039). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:100) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (dilution 1:50) was used as secondary antibody. |
|
Immunohistochemical analysis of paraffin embedded Thyroid Carcinoma tissue -with anti BRaf(V600E) monoclonal antibody (Cat. # 26039). Tissue samples were fixed with paraffin. Samples were then incubated with primary antibody (1:300) overnight at 4°C. |
| Related Products | |||
| Catalog# | Name | Size | Price |
| 21271 | Anti BRaf Rabbit Polyclonal Antibody | 100 μL | ￥2600 |
| 26129 | Anti BRaf Mouse Monoclonal Antibody | 100 μL | ￥2800 |
| 26109 | Anti BRaf(N581S) Mouse Monoclonal Antibody | 100 μL | ￥4800 |
| 26039 | Anti BRaf(V600E) Mouse Monoclonal Antibody | 100 μL | ￥4800 |
| 26110 | Anti BRaf(V600K) Mouse Monoclonal Antibody | 100 μL | ￥4800 |
| 26184 | Anti BRaf(V600D) Mouse Monoclonal Antibody | 100 μL | ￥4800 |
| 26182 | Anti BRaf(V600R) Mouse Monoclonal Antibody | 100 μL | ￥4800 |
| 26196 | Anti BRaf(G469E) Mouse Monoclonal Antibody | 100 μL | ￥4800 |
| 26199 | Anti BRaf(V600A) Mouse Monoclonal Antibody | 100 μL | ￥4800 |
| 26462 | Anti BRAF(V600M) Mouse Monoclonal Antibody | 100 μL | ￥4800 |
影响因子：PLOS ONE IF=3.7
应用范围：IF（作为临床诊断工具的验证 CTC）
种属：Human (黑色素瘤）
影响因子：Journal of Surgical Research IF=2.2
应用范围：IHC（作为临床诊断工具的验证与VE1 同等）
种属：Human
影响因子：Clinical Endocrinology IF=3.2
应用范围：IHC（作为临床诊断工具的验证）
种属：Human（甲状腺瘤）
影响因子：Tumor Biology
应用范围：IHC（作为临床诊断工具的验证）
种属：Human（黑色素瘤）
影响因子：Modern Pathology IF=7.5
应用范围：IHC（作为临床诊断工具的验证）
种属：Human（黑色素瘤，甲状腺瘤，结直肠癌）
